#Living image perkin elmer software#
#Living image perkin elmer plus#

Wide range of oil, glycerol and water immersion objectives.

Inverted Zeiss Axio Observer Z1 microscope.

Images were collected using a Zeiss LSM 510 confocal microscope.Д«enjamin Boettner and Ulrike Gaul (Rockefeller University) Spinning disk confocal microscope for live cell imaging (Zeiss/Perkin-Elmer/Spectral Applied) вЂ“ вЂњBuntyвЂќ Cell outlines in the Drosophila embryo, as labeled by adherens junction markers.

Weatherstation environmental chamber вЂ“ can be set at temperatures from ambient temperature to 37вЊД¦.

вЂњUltimate FocusвЂќ вЂ“ hardware-based autofocus system for long-term timelapse imaging without focal drift.

#Living image perkin elmer plus#

All the features of the above system plus a FRAP/photoactivation module with 405 and 488 lasers.2nd DeltaVision Image Restoration Microscope (Applied Precision) вЂ“ вЂњTottyвЂќ Warner Instruments stagetop imaging chambersДҐ.Insight SSI 7 color solid state illumination system.Extremely precise stage allowing multiple point visiting and image stitching.Separate excitation and emission filter wheels (necessary for FRET).Filter sets for DAPI, CFP, GFP/FITC, YFP, Rhodamine, AlexaFluor 594/mCherry and Cy5.40x, 60x and 100x oil objectives, 60x water objective, 60x and 100x Silicone oil objectives, 20x dry objective, plus 1.5x optovar.DeltaVision Image Restoration Microscope (Applied Precision) вЂ“ вЂњWallaceвЂќ Solent Scientific environmental chamber for temperature regulationД¤.

#Living image perkin elmer software#

MetaMorph acquisition software (includes MDA).Prior XYZ piezo stage (200 microns z-travel) for multiple point visiting during timelapse acquisitions.Hamamatsu Orca ER B/W digital camera and QI Click color digital camera.Filter sets for DAPI, CFP, GFP, YFP, RFP and Cy5.10x, 20x, 40x, 60x and 100x objective lenses, some suitable for use with plastic as well as glass dishes/plates.Olympus IX71 inverted microscope with phase contrast optics.Mary Miller and Frederick Cross (Rockefeller University) Images were captured and deconvolved using the DeltaVision image restoration microscope, allowing fine structures to be revealed within these small cells. Inverted microscope for long-term time-lapse, fluorescent and phase contrast imaging of cells or embryos (Olympus) вЂ“ вЂњWendoleneвЂќ Yeast cells labeled for actin (green), Cln2 (red) and nuclei (blue). Nikon Eclipse TS100 inverted microscopeДЈ.Inverted fluorescence microscope for checking fluorescent protein expression levels in tissue culture cells (Nikon) MetaVue acquisition software (Molecular Devices)Дў.Spot Insight QE color digital camera for brightfield (and fluorescence) imaging or Hamamatsu Orca ER B/W digital camera for sensitive fluorescence imaging.Brightfield and Differential Interference Contrast (DIC).Filter sets for DAPI, CFP, GFP, AF 488, YFP, Texas Red, Cy5.Wide-field fluorescence/brightfield/DIC microscope (Zeiss) вЂ“ вЂњEdwinaвЂќ Light microscopy Microscopes (DWB room 201, 202 and 203)Д¬urrent equipment in the light microscope facility includes:ДЎ.